| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| 10_FS_1_2Aligned.sortedByCoord.out.bam | 23,868,599 | 0 | 22-76 | 46 |
| 10_FS_1Aligned.sortedByCoord.out.bam | 22,556,636 | 0 | 23-76 | 46 |
| 11__-_5_2Aligned.sortedByCoord.out.bam | 23,202,507 | 0 | 23-76 | 45 |
| 11__-_5Aligned.sortedByCoord.out.bam | 21,951,190 | 0 | 23-76 | 45 |
| 12__-_4_2Aligned.sortedByCoord.out.bam | 24,120,299 | 0 | 23-76 | 45 |
| 12__-_4Aligned.sortedByCoord.out.bam | 22,807,843 | 0 | 23-76 | 45 |
| 13__-_3_2Aligned.sortedByCoord.out.bam | 18,641,066 | 0 | 24-76 | 45 |
| 13__-_3Aligned.sortedByCoord.out.bam | 17,605,122 | 0 | 23-76 | 45 |
| 14__-_2_2Aligned.sortedByCoord.out.bam | 26,437,496 | 0 | 23-76 | 45 |
| 14__-_2Aligned.sortedByCoord.out.bam | 25,045,051 | 0 | 23-76 | 45 |
| 15__-_1_2Aligned.sortedByCoord.out.bam | 28,019,239 | 0 | 23-76 | 45 |
| 15__-_1Aligned.sortedByCoord.out.bam | 26,516,612 | 0 | 23-76 | 45 |
| 1_FAD_5_2Aligned.sortedByCoord.out.bam | 24,871,513 | 0 | 23-76 | 45 |
| 1_FAD_5Aligned.sortedByCoord.out.bam | 23,538,193 | 0 | 24-76 | 45 |
| 2_FAD_4_2Aligned.sortedByCoord.out.bam | 24,398,666 | 0 | 24-76 | 46 |
| 2_FAD_4Aligned.sortedByCoord.out.bam | 23,157,372 | 0 | 23-76 | 46 |
| 3_FAD_3_2Aligned.sortedByCoord.out.bam | 24,672,290 | 0 | 22-76 | 45 |
| 3_FAD_3Aligned.sortedByCoord.out.bam | 23,380,344 | 0 | 23-76 | 45 |
| 4_FAD_2_2Aligned.sortedByCoord.out.bam | 23,388,035 | 0 | 23-76 | 45 |
| 4_FAD_2Aligned.sortedByCoord.out.bam | 22,123,347 | 0 | 22-76 | 45 |
| 5_FS_2_2Aligned.sortedByCoord.out.bam | 22,457,813 | 0 | 23-76 | 45 |
| 5_FS_2Aligned.sortedByCoord.out.bam | 21,194,114 | 0 | 23-76 | 45 |
| 6_FAD_1_2Aligned.sortedByCoord.out.bam | 25,406,590 | 0 | 23-76 | 46 |
| 6_FAD_1Aligned.sortedByCoord.out.bam | 24,056,971 | 0 | 23-76 | 46 |
| 7_FS_5_2Aligned.sortedByCoord.out.bam | 27,134,036 | 0 | 23-76 | 45 |
| 7_FS_5Aligned.sortedByCoord.out.bam | 25,628,465 | 0 | 23-76 | 45 |
| 8_FS_4_2Aligned.sortedByCoord.out.bam | 25,057,514 | 0 | 23-76 | 45 |
| 8_FS_4Aligned.sortedByCoord.out.bam | 23,784,746 | 0 | 23-76 | 45 |
| 9_FS_3_2Aligned.sortedByCoord.out.bam | 22,482,650 | 0 | 24-76 | 45 |
| 9_FS_3Aligned.sortedByCoord.out.bam | 21,210,412 | 0 | 23-76 | 45 |
Library Sizes ranged between 17,605,122 and 28,019,239 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Transcriptome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Transcriptome
Total overrepresented sequences for each library
## The Overrepresented_sequences module was empty in:
## 10_FS_1_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 10_FS_1Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 11__-_5_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 11__-_5Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 12__-_4_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 12__-_4Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 13__-_3_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 13__-_3Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 14__-_2_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 14__-_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 15__-_1_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 15__-_1Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 1_FAD_5_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 1_FAD_5Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 2_FAD_4_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 2_FAD_4Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 3_FAD_3_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 3_FAD_3Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 4_FAD_2_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 4_FAD_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 5_FS_2_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 5_FS_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 6_FAD_1_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 6_FAD_1Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 7_FS_5_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 7_FS_5Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 8_FS_4_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 8_FS_4Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 9_FS_3_2Aligned.sortedByCoord.out_fastqc.zipThe Overrepresented_sequences module was empty in:
## 9_FS_3Aligned.sortedByCoord.out_fastqc.zip
## No overrepresented sequences were detected by FastQC
Universal Adapter Content
## The Kmer_Content module was missing from:
## 10_FS_1_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 10_FS_1Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 11__-_5_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 11__-_5Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 12__-_4_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 12__-_4Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 13__-_3_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 13__-_3Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 14__-_2_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 14__-_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 15__-_1_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 15__-_1Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 1_FAD_5_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 1_FAD_5Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 2_FAD_4_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 2_FAD_4Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 3_FAD_3_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 3_FAD_3Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 4_FAD_2_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 4_FAD_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 5_FS_2_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 5_FS_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 6_FAD_1_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 6_FAD_1Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 7_FS_5_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 7_FS_5Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 8_FS_4_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 8_FS_4Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 9_FS_3_2Aligned.sortedByCoord.out_fastqc.zipThe Kmer_Content module was missing from:
## 9_FS_3Aligned.sortedByCoord.out_fastqc.zip
## No Kmers were detected by FastQC